Identification of extensive cellular diversity and maturation of active neuronal networks in long-term cultures of human brain organoids. Homo sapiens

We analyzed gene expression in over 80,000 individual cells isolated from 31 human whole-brain organoids that has developed for 3-6 months. We find that organoids can generate a broad diversity of cells, which we show are related to known endogenous classes, including subpopulations of neurons and progenitors of the cerebral cortex Overall design: Single cell Droplet sequencing of human cerebral organoid

本研究对31株培养3~6个月的人类全脑类器官（whole-brain organoids）中分离得到的8万余个单个细胞开展了基因表达分析。研究发现，此类类器官可产生广泛多样的细胞类群，经证实这些细胞与已知的内源性细胞类群存在关联，其中包括大脑皮层（cerebral cortex）神经元亚群及神经前体细胞。实验设计概述：人类大脑类器官的单细胞液滴测序（Single cell Droplet sequencing）