Whole transcriptome analysis of mucosal melanoma. Whole transcriptome analysis of mucosal melanoma

This study reports bulk total RNA sequencing from melanoma patient-derived xenograft (PDX) tumors. The goal is to determine transcriptomic differences across melanomas of distinct subtypes including cutaneous, acral, mucosal, and unknown primary. Fresh surgical specimens used for deriving PDX models were obtained from the University of Colorado Skin Cancer Biorepository under protocol COMIRB #05-0309. Snap frozen tumors were used for RNA sequencing.

本研究针对黑色素瘤患者来源异种移植（patient-derived xenograft，PDX）肿瘤开展批量总RNA测序，旨在明确不同亚型黑色素瘤（含皮肤型、肢端型、黏膜型及原发灶不明型）的转录组差异。本研究构建PDX模型所用的新鲜手术标本取自科罗拉多大学皮肤癌生物样本库，相关实验方案编号为COMIRB #05-0309，测序所用组织为经速冻处理的肿瘤样本。