Orphan box C/D snoRNAs guide rRNA base acetylations in yeast

Box C/D snoRNAs snR4 and snR45, whose role was unknown, guide 18S rRNA acetylations by Kre33 in yeast. UV-crosslinking (CRAC) was used to determine binding sites of Kre33 on RNA. Duplicate data-sets of cDNA reads of RNAs UV-crosslinked to Kre33-HTP in vivo using the CRAC protocol. Cells were in mid-log phase when irradiated, immediately collected by filtration and frozen

功能尚不明确的盒C/D型小核仁RNA（Box C/D snoRNAs）snR4与snR45，可在酵母中引导Kre33酶对18S核糖体RNA（18S rRNA）进行乙酰化修饰。本研究采用紫外交联（CRAC）技术，确定了Kre33在RNA分子上的结合位点。本数据集包含两组重复的cDNA读段数据，对应采用CRAC实验流程在活体内与Kre33-HTP融合蛋白发生紫外交联的RNA的互补DNA测序读段。实验中，细胞处于对数中期时接受紫外照射，随后立即通过过滤收集并冷冻保存。