Global transcriptional response of E. coli to the presence and absence of bile salts. Escherichia coli

Unlike many other types of diarrheagenic bacteria that act primarily in the small intestine, O157:H7 expresses virulence primarily in the large intestine. In this study, microarray analysis is employed to examine the transcriptional response of O157:H7 to bile treatment, to gain insight into how bile affects virulence and whether bile might be temporally defending the small intestine against virulence by these bacteria. Keywords: Expression profiling of two different growth conditions Overall design: Two groups of three replicates were used: E.coli O157:H7 grown in Luria broth with or without 0.8% bile salts

与多数主要作用于小肠的致泻性细菌不同，大肠杆菌O157:H7（E. coli O157:H7）的致病力主要在大肠中表达。本研究采用微阵列（microarray）分析技术，检测大肠杆菌O157:H7经胆汁处理后的转录应答，以深入解析胆汁对其致病力的调控机制，并探究胆汁是否可通过时间依赖性方式抵御小肠受此类细菌的侵袭。关键词：两种不同生长条件下的表达谱分析。实验设计：设置两组实验，每组包含3次生物学重复：将大肠杆菌O157:H7分别在添加0.8%胆汁盐与未添加胆汁盐的LB培养基（Luria broth）中培养。