FOXM1 in Early Human Squamous Cell Carcinoma Oncogenesis and it Is Enhanced by Nicotine during Malignant Transformation. Homo sapiens

Genome-wide SNP profilling for loss of heterozygosity (LOH) during FOXM1B-induced malignant transformation in a human premalignant oral keratinocyte line SVpgC2a. Keywords: oral cancer, keratinocytes, head and neck squamous cell carcinoma, FOXM1, genomic instability, SNP array, loss of heterozygosity, malignant transformation Overall design: Eight FOXM1B-induced malignant clones (SVFN1-8) that grew in soft agar were grown as individual cell lines and gDNA harvested from each clone for SNP array analysis compared to wild-type non-transformed SVpgC2a as control to obtain loss of heterozygosity profiles.

本数据集针对人癌前口腔角质形成细胞系SVpgC2a中FOXM1B诱导的恶性转化过程中的杂合性缺失（loss of heterozygosity, LOH）开展全基因组SNP分型（Genome-wide SNP profiling）。关键词：口腔癌、角质形成细胞、头颈部鳞状细胞癌、FOXM1、基因组不稳定性、SNP芯片（SNP array）、杂合性缺失、恶性转化。总体设计：将8个经FOXM1B诱导、可在软琼脂中形成克隆的恶性克隆（SVFN1-8）分别建立为独立细胞系，提取各克隆的基因组DNA（genomic DNA, gDNA）进行SNP芯片分析，并以未转化的野生型SVpgC2a作为对照，以此获取杂合性缺失图谱。