Human endometrial organoids

Human endometrial organoid cultures were established from the decidual samples. We analyzed the global expression profiles of the endometrial organoids. Next, we determined the global expression patterns upon estrogen treatment and compared them to untreated controls. Overall design: We employed RNA-seq to analyze the global expression profiles of endometrial organoids (EORG-1, EORG-2, EORG-3, EORG-C; 3 replicates each). Next, we determined the global expression patterns upon estrogen treatment (3 replicates: EORG-E) and compared them to untreated controls (3 replicates: EORG-C).

本研究从蜕膜样本中构建了人子宫内膜类器官培养体系。我们对子宫内膜类器官的全局基因表达谱进行了分析。随后，我们检测了雌激素处理后的全局基因表达模式，并与未处理的对照组进行了比较。
整体实验设计：我们采用RNA测序（RNA-seq）技术分析子宫内膜类器官的全局基因表达谱，涉及EORG-1、EORG-2、EORG-3、EORG-C四个样本组，每组设置3次生物学重复。随后，我们检测了雌激素处理组（3次生物学重复，样本编号为EORG-E）的全局基因表达模式，并与未处理的对照组（3次生物学重复，样本编号为EORG-C）进行了对比。