Expression data from early zebrafish embryo

Zebrafish pou5f3 regulates various developmental processes, including neurogenesis and brain formation. We used microarrays to detail the global program of gene expression regulating isthmus development around the end of gastrulation. To this aim, we employed transgenic (Tg) embryos harboring a heat-inducible dominant-interference pou5f3 gene (en-pou5f3) that functionally suppresses pou5f3 (Tg(hsp-en-pou5f3)). en-pou5f3 was induced by heat shock in Tg embryos at 90% epiboly or 3-somite stage (3-ss). The transcriptomes were compared on Affymetrix microarrays to know the gene profiles specific to each developmental stage.

斑马鱼pou5f3可调控包括神经发生、脑形成在内的多种发育过程。本研究利用微阵列技术，详细解析了原肠胚期末期调控脑峡部发育的全局基因表达程序。为达成该研究目标，我们采用了品系为Tg(hsp-en-pou5f3)的转基因（Tg）胚胎，该胚胎携带可功能性抑制pou5f3的热诱导型显性干扰pou5f3基因（en-pou5f3）。在该转基因胚胎发育至90%下包期或3体节期（3-ss）时，通过热休克诱导en-pou5f3的表达。随后通过Affymetrix微阵列对各组转录组进行比较分析，以获取各发育阶段特异性的基因表达谱。