Nucleosome occupancy as a novel chromatin parameter for replication origin functions. Saccharomyces cerevisiae

This study defines nucleosome occupancy as a novel parameter for regulating origin activities. Nucleosome occupancy at origins was assessed using histone H3 ChIP-seq during G1 and G2M. Origin activity was determined using BrdU ChIP-seq. Overall design: Nucleosome occupancy at origins was assessed using histone H3 ChIP-seq during G1 and G2M.

本研究将核小体占位（nucleosome occupancy）定义为调控复制起始位点活性的新型参数。在G1期与G2/M期，采用组蛋白H3染色质免疫沉淀测序（ChIP-seq）评估复制起始位点处的核小体占位水平。复制起始位点活性通过溴脱氧尿苷（BrdU）染色质免疫沉淀测序（ChIP-seq）进行测定。实验设计概述：在G1期与G2/M期，采用组蛋白H3染色质免疫沉淀测序（ChIP-seq）评估复制起始位点处的核小体占位水平。