Description of amino acid substitutions in de novo produced site-directed chlamydomonas D1 mutants.

The reported set of D1 mutations could account for the radiation tolerance previously found in D1 random mutants surviving neutron and proton exposure, and was selected to produce the corresponding site-directed mutants.
aThe side chain polarity is presented according to [53], the hydropathy index is cited as in [54], the amino acids reactivity classes are reported in Table 2.

已报道的一组D1突变可解释此前在经受中子与质子辐照后存活的D1随机突变体中观测到的辐射耐受性，且该组突变被用于制备对应的定点突变体。注：侧链极性依据文献[53]确定，亲水性指数引自文献[54]，氨基酸反应性分类详见表2。