Effects of mixed strains of rhizopus oryzae and lactobacillus on corn meal fermentation

Abstract As long as they are provided in appropriate proportions, probiotics can be beneficial to the host. These bacteria are increasingly used in food to balance intestinal microbiota and relieve gastrointestinal disorders. However after traveling through the gastrointestinal (GI) tract, surviving probiotic bacteria comprise 10 to 30 % of this population. It is a probiotic bacterium found in many probiotic foods. As a result of its inability to hydrolyze proteins and macromolecule carbs, L. acidophilus grows poorly in cereal products. The goal of the present investigation was a synbiotic beverage made from corn mash and Rhizopus oryzae-fermented corn mash. Starting culture concentration is one such element. Milk powder and Corn mash that had been fermented with Rhizopus oryzae were both researched in depth. Fermented cornflour with R. oryzae had just enough nutrients to support L. acidophilus' survival, but not its development. The proliferation of Lactobacillus acidophilus was not improved by adding sugar (1 or 2 %, w/v). However, once milk powder (1 % or 2 %, w/v) was put in, L. acidophilus developed rapidly. After 10 hours of fermentation using 5.5 % Rhizopus oryzae -fermented corn mash and 2 % Cell counts for skim milk powder were about. 9.0 log CFU/mL. During fermentation, the content of -glucans (approximately 781 mg/L) did not change considerably.

摘要 只要配比适宜，益生菌（probiotics）便可对宿主发挥有益作用。此类细菌正愈发广泛地应用于食品工业，用于调节肠道菌群（intestinal microbiota）平衡并缓解胃肠道病症（gastrointestinal disorders）。然而，经过胃肠道（gastrointestinal tract，简称GI）转运后，存活的益生菌仅占总菌群的10%~30%。嗜酸乳杆菌（L. acidophilus）是一类广泛存在于多款益生菌食品中的益生菌菌株；由于其无法水解蛋白质与大分子碳水化合物，嗜酸乳杆菌在谷物类制品中生长状况不佳。本研究旨在开发一款由玉米醪液与米根霉（Rhizopus oryzae）发酵玉米醪液制备的合生元（synbiotic）饮品。接种起始浓度即为其中一项关键影响因素，研究团队对脱脂奶粉与米根霉发酵玉米醪液分别开展了深入探究。米根霉发酵玉米粉仅能提供满足嗜酸乳杆菌存活所需的营养物质，却无法支撑其增殖；添加1%或2%（w/v，质量体积比）的蔗糖并未促进嗜酸乳杆菌的增殖。但当添加1%或2%（w/v）的脱脂奶粉后，嗜酸乳杆菌可实现快速增殖。当采用5.5%米根霉发酵玉米醪液与2%脱脂奶粉进行发酵时，发酵10小时后嗜酸乳杆菌的活菌数可达约9.0 log CFU/mL（菌落形成单位/毫升）。发酵过程中，β-葡聚糖（β-glucans）的含量（约781 mg/L）未出现显著变化。