PMACS-11.

<b>Project title</b>: PMACS-11<b>Grant Information:</b> AHA Grant 9TPA34910007, Regulatory role of pericardial macrophages in myocardial infarction<b>Project Description:</b>· Animal Model: Myocardial Infarction· Animal Age: 8-12 Weeks· Animal Sex: Male· Animal Strain: C57B6/J· Number: 9 mice· Groups: 5 chlodronate liposome injection, 4 empty liposome injections, all infarctions· Experimental Conditions: Mice were given MI via LAD ligation alongside intrapericardial injection of chlodronate loaded liposomes. Animals were sacrificed at day 21 post-infarction or sham surgery. Animals were analyzed via EKG and then hearts were harvested for flow cytometry and Mason’s Trichrome histology<b>Code/Scripts: </b>N/A<b>Licenses: </b>N/A<br><br><br><br><br><br><br>

<b>项目标题</b>：PMACS-11<br><b>资助信息</b>：美国心脏协会（American Heart Association, AHA）资助项目编号9TPA34910007，研究主题为"心包巨噬细胞在心肌梗死中的调控作用"<br><b>项目说明</b>：<br>· 动物模型：心肌梗死（Myocardial Infarction）<br>· 动物周龄：8~12周<br>· 动物性别：雄性<br>· 动物品系：C57B6/J<br>· 实验动物数量：9只小鼠<br>· 分组情况：5只接受氯膦酸脂质体（clodronate liposome）注射，4只接受空白脂质体注射，所有小鼠均构建心肌梗死模型<br>· 实验处理：通过左前降支（Left Anterior Descending, LAD）结扎构建心肌梗死模型，同时心包腔内注射负载氯膦酸的脂质体；分别于梗死造模或假手术后第21天处死动物。先通过心电图（Electrocardiogram, EKG）对动物进行检测，随后摘取心脏进行流式细胞术（flow cytometry）及马松三色（Mason’s Trichrome）组织学分析<br><b>代码/脚本</b>：无<br><b>许可协议</b>：无