mRNA-Seq of Danio rerio exposed to different concentrations of fungicide epoxiconazole against untreated control groups

In the present study transcriptome analysis was employed to investigate the early molecular responses to exposure to epoxiconazole (CAS 133855-98-8). Zebrafish embryos were exposed to epoxiconazole according to OECD guidelines (OECD test No. 236). At the end of exposure time (96 hours), simultaneous RNA and protein extraction from 10 embryos was performed using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina NovaSeq 6000 system System and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally, potential substance specific biomarker candidates were selected based on the differential expression patterns and the biological functions investigation of the detected DEGs.

本研究采用转录组分析（transcriptome analysis）技术，探究斑马鱼胚胎暴露于环氧康唑（epoxiconazole，CAS 133855-98-8）后的早期分子应答。实验依据经济合作与发展组织（OECD）第236号测试指南（OECD test No. 236）的要求，对斑马鱼胚胎进行环氧康唑暴露处理。暴露时长为96小时，处理结束后使用Macherey & Nagel品牌的RNA/蛋白提取试剂盒，从10枚胚胎中同步提取RNA与蛋白质。提取获得的RNA样本通过Illumina NovaSeq 6000测序系统完成测序，所得测序数据经生物信息学分析流程，完成检测基因序列的鉴定与计数，并进一步开展差异基因表达分析。最终，基于检测到的差异表达基因（differentially expressed genes, DEGs）的表达模式与生物学功能分析，筛选得到该物质潜在的特异性候选生物标志物。