RNA-Seq Analysis of the Human Surfactant Air-Liquid Interface Culture Reveals Alveolar Type II Cell-like Transcriptome

In this study we investigate the effects of SALI culture conditions on the transcriptional profile of the constituent H441 cells. We further analyze the transcriptomics of the model in the context of surfactant metabolism and the disease phenotype through SFTPB-knockout SALI cultures. By comparing the gene expression profile of SALI cultures with that of human lung parenchyma obtained via single-cell RNA-sequencing, we found that SALI cultures are remarkably similar to human alveolar type II cells implying clinical relevance of the platform as a non-diseased lung model. mRNA profiles of wild-type and SFTPB KO H441 cells grown in submerged and SALI culture conditions

本研究探究了SALI培养条件对体系内H441细胞转录谱的影响。本研究进一步借助SFTPB基因敲除（SFTPB-knockout）的SALI培养体系，围绕肺表面活性物质代谢与疾病表型维度，分析该模型的转录组学特征。通过将SALI培养体系的基因表达谱与单细胞RNA测序（single-cell RNA-sequencing）获取的人肺实质样本的基因表达谱进行对比分析，我们发现SALI培养体系与人肺泡II型细胞的转录特征高度相似，这表明该平台作为非病变肺脏模型具备临床应用相关性。本数据集涵盖浸没培养与SALI培养条件下培养的野生型及SFTPB敲除（SFTPB KO）H441细胞的mRNA表达谱。