cRel Expression Regulates Distinct Transcriptional and Functional Profiles Driving Fibroblast Matrix Production in Systemic Sclerosis

The scope of this project is to investigate transcriptional differences bewteen wild type and Rel-/- fibroblasts under basal conditions. Mouse fibroblasts were isolated via explant culture from skin and lungs of un-challenged mice. Male C57BL/6J and Rel -/- mice (housed in IVC cages) aged 8-10 weeks, mice were euthanized by cervical dislocation and skin (dorsal area) and lung tissues were extracted

本项目的研究范畴为探究基础条件下野生型（wild type）与Rel-/-成纤维细胞（fibroblasts）之间的转录差异。实验所用小鼠成纤维细胞通过外植体培养（explant culture）法，从未受外界干预的健康小鼠的皮肤与肺组织中分离得到。选取8~10周龄的雄性C57BL/6J小鼠及Rel-/-基因敲除小鼠，饲养于IVC笼（IVC cages）中，通过颈椎脱臼法实施安乐死后，提取其背侧区域皮肤及肺组织。