Ploidy estimation for an artificially combined wheat dataset.

Reads from the large arms of chromosomes 5A, 5B and 5D were pooled, assembled and used for ploidy estimation. Only contigs with average coverage of 10X or above, and for which the individual ploidy in a given subgenome was estimated to be one were considered.aBased on BLAST alignments to the individual assemblies.Ploidy estimation for an artificially combined wheat dataset.将来自5A、5B和5D三条染色体长臂的测序读段（reads）进行混合与组装，用于倍性评估。仅保留平均覆盖度不低于10X，且指定亚基因组中的个体倍性被评估为1的重叠群（contigs）。[a] 基于与各独立组装结果的BLAST比对。本倍性评估针对人工组合小麦数据集开展。