Chronic inflammation is a risk factor for gastric carcinogenesis after Helicobacter pylori eradication [miRNA]

Even after endoscopic treatment of early gastric adenocarcinoma (GAC) and eradication of Helicobacter pylori (H. pylori), some patients develop a metachronous recurrence (MR), the mechanism of which is still unknown. To elucidate the mechanism and risk factors for MR, we analyzed gene expression at multiple locations of the gastric mucosa, considering the heterogeneity of gastric mucosal damage caused by H. pylori infection and investigated the mechanism and risk factors for MR. Five patients with MR (MR group) and five patients without MR (control) after early GAC treatment and eradication of H. pylori were included. In each case, mucosal tissue was collected as biopsy specimens from four sites in the stomach. In each patient, one specimen was obtained from the lesser curvature of the antrum (LA) and the greater curvature of the antrum (GA), both within 2−3 cm from the pylorus, middle portion of the lesser curvature of the body between the cardia and angle (LB), and middle portion of the greater curvature of the corpus (GB).mRNA sequencing and microRNA(miRNA) sequencing were performed.

即使在早期胃腺癌（gastric adenocarcinoma, GAC）接受内镜治疗并根除幽门螺杆菌（Helicobacter pylori, H. pylori）后，部分患者仍会发生异时性复发（metachronous recurrence, MR），其具体发病机制目前尚未明确。为阐明异时性复发的发病机制与危险因素，本研究充分考虑幽门螺杆菌感染所致胃黏膜损伤的异质性，对胃黏膜多部位的基因表达谱进行了分析，并进一步探究了异时性复发的相关机制与危险因素。本研究纳入了早期胃腺癌内镜治疗且根除幽门螺杆菌后出现异时性复发的患者5例（复发组），以及同期未出现复发的患者5例（对照组）。所有受试者均从胃内4个部位采集黏膜活检组织标本：分别为距幽门2~3 cm范围内的胃窦小弯侧（LA）、胃窦大弯侧（GA），贲门与胃角之间的胃体小弯中段（LB），以及胃体大弯中段（GB）。随后对所有标本进行了信使RNA（mRNA）测序及微小RNA（miRNA）测序。