CIL:37034, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

This is IMAGE #1 (day 1) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged daily over 3 days and shows the development of the dendritic arbor and axon over time.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

本图像为图像#1（第1天），来源于47期白化品系非洲爪蟾（Xenopus laevis）蝌蚪中枢神经系统（Central Nervous System, CNS）视顶盖（optic tectum）中的发育中神经元。该细胞被连续3天每日成像，完整记录了其树突分支与轴突随时间推移的发育过程。
在图像采集前约24小时，通过体内电穿孔法（in vivo electroporation）将编码增强型绿色荧光蛋白（enhanced Green Fluorescent Protein, eGFP）的质粒DNA转染入该细胞。将完整活体蝌蚪麻醉后固定于盖玻片下方，通过其透明的皮肤，使用定制双光子显微镜（two-photon microscope）直接采集得到本图像。