LncRNA and mRNA Sequencing Analysis of Left Testis in Experimental Varicocele Rats Treated with Morinda officinalis Polysaccharide. LncRNA and mRNA Sequencing Analysis of Left Testis in Experimental Varicocele Rats Treated with Morinda officinalis Polysaccharide

Purpose: Next-generation sequencing (NGS) was performed to illustrate the genetic molecular mechanism underlying varicocele (VC) pathogenesis and Morinda officinalis polysaccharide (MOP) repair effect. Methods: The male Sprague–Dawley (SD) rats (about 6-7 weeks old and 200±10g weight) were divided into three groups: 1) control group (Control); 2) experimental varicocele group (VC); 3) 300 mg kg-1 MOP administration group (VC+MOP). The next-generation RNA sequencing were performed to identify the differentially-expressed mRNA and lncRNA in left testicular tissue between each two compared group. Results: The result show that a total of 144 mRNAs and 63 LncRNAs, 63 mRNAs and 148 LncRNAs , 173 mRNAs and 54 LncRNAs were found differentially expressed between compared group VC vs. Control, VC+MOP vs. VC, and VC+MOP vs. Control respectively. Conclusions: Our study try to offer a indication for novel diagnose marker and therapeutic method, as well as provide feasible research orientations for further study. Overall design: Left testicular mRNA and lncRNA profiles of experimental varicocele rats treated with Morinda officinalis polysaccharide were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000.

研究目的：采用下一代测序（next-generation sequencing, NGS）技术，阐明精索静脉曲张（varicocele, VC）发病的遗传分子机制及巴戟天多糖（Morinda officinalis polysaccharide, MOP）的修复效应。
实验方法：选取6~7周龄、体重200±10g的雄性Sprague-Dawley（SD）大鼠，随机分为3组：1）对照组（Control组）；2）实验性精索静脉曲张组（VC组）；3）300 mg·kg⁻¹巴戟天多糖给药组（VC+MOP组）。采用下一代RNA测序技术，对各组大鼠左侧睾丸组织中的差异表达信使RNA（messenger RNA, mRNA）与长链非编码RNA（long non-coding RNA, lncRNA）进行组间两两比较分析。
实验结果：经两两组间对比，VC组与对照组（VC vs. Control）、VC+MOP组与VC组（VC+MOP vs. VC）、VC+MOP组与对照组（VC+MOP vs. Control）分别筛选得到144个mRNA及63个lncRNA、63个mRNA及148个lncRNA、173个mRNA及54个lncRNA的差异表达转录本。
研究结论：本研究可为精索静脉曲张的新型诊断标志物与治疗方案提供理论参考，同时为后续相关研究指明可行方向。
整体实验设计：采用Illumina HiSeq 4000测序平台进行深度测序，对经巴戟天多糖处理的实验性精索静脉曲张大鼠的左侧睾丸mRNA及lncRNA表达谱进行3次生物学重复检测。