RNA Sequencing of induced pluripotent stem cell derived motor neurons from C9orf72 hexanucleotide repeat expansion carriers and controls

Transcriptomic signature of the C9orf72 hexonucleotide repeat expansion in induce pluripotent stem cell derived neurons. Overall design: iPSC-MNs for RNA sequencing were harvested on DIV 39 from 3 control lines and 4 patient lines (from 3 individuals, with one providing two clones) from ALS patients carrying the C9orf72 hexonucleotide repeat expansion.

C9orf72六核苷酸重复扩增在诱导多能干细胞（induced pluripotent stem cell, iPSC）分化神经元中的转录组特征。实验整体设计：针对携带C9orf72六核苷酸重复扩增的肌萎缩侧索硬化症（Amyotrophic Lateral Sclerosis, ALS）患者来源的诱导多能干细胞运动神经元（iPSC-MNs），于体外培养第39天（days in vitro, DIV 39）收集样本进行RNA测序，样本涵盖3株对照细胞系与4株患者来源细胞系（取自3名个体，其中1名个体提供2个克隆株）。