Response of expanded human conventional and regulatory T cells to CD28 or TNFR2 costimulation [Exp2]. Response of expanded human conventional and regulatory T cells to CD28 or TNFR2 costimulation [Exp2]

Immune responses depend on a dynamic balance between the opposing activities of conventional (Tconv) and regulatory (Treg) CD4+ T cells. While receptor-targeted approaches have been developed based on their modulation of Tconv cells, Tconv and Treg cells share many costimulatory receptors. We aim to determine key differential signaling events downstream of costimulation to find opportunities for selective manipulation of Tconv or Treg cells. This data set includes the transcriptomes of expanded human Tconv and Treg cells that were treated with anti-CD3, anti-CD3/CD28 or anti-CD3/TNFR2 agonistic mAbs for 24 hours. Overall design: Whole transcriptome analysis of expanded human conventional and regulatory T cells that were treated with anti-CD3, anti-CD3/CD28 or anti-CD3/TNFR2 agonistic mAbs (three healthy blood donors).

免疫应答依赖于经典CD4+T细胞（conventional CD4+ T cells，简称Tconv）与调节性CD4+T细胞（regulatory CD4+ T cells，简称Treg）之间相互拮抗的活性所维持的动态平衡。尽管基于调控经典CD4+T细胞已开发出受体靶向疗法，但Tconv与Treg细胞共享大量共刺激受体。本研究旨在明确共刺激信号下游的关键差异信号事件，以找到选择性调控Tconv或Treg细胞的潜在策略。本数据集涵盖经扩增的人Tconv与Treg细胞的转录组数据，这些细胞分别经抗CD3、抗CD3/CD28及抗CD3/TNFR2激动性单克隆抗体处理24小时。整体实验设计：对经抗CD3、抗CD3/CD28及抗CD3/TNFR2激动性单克隆抗体处理的扩增人经典与调节性T细胞开展全转录组分析，样本取自3名健康献血者。