Gene Expression Response to Interferon Treatment at 12h and 24 in A549 cells. Homo sapiens

The goal of this experiment was to use global gene expression profiling to understand human lung epithelial cellular responses to interferon treatment. Overall design: A549 cells were treated individually with IFN-α (10 ng ml−1; Sigma I4276), IFN-β (500 U ml−1; Sigma I4151) and IFN-γ (10 ng ml−1; Sigma I32265) or mock-treated. At 12 and 24 h post-treatment, cells were harvested (n = 3 wells per condition) and total RNA was extracted and analysed by microarray. Individual well replicates were pooled for each array by combining equal masses of total RNA (n=1 array per time point for each treatment and control).

本实验旨在通过全基因组基因表达谱分析，探究人类肺上皮细胞对干扰素处理的应答反应。实验设计如下：将A549细胞分别用干扰素-α（IFN-α，10 ng·ml⁻¹；Sigma I4276）、干扰素-β（IFN-β，500 U·ml⁻¹；Sigma I4151）与干扰素-γ（IFN-γ，10 ng·ml⁻¹；Sigma I32265）单独处理，同时设置空白对照处理组。分别于处理后12小时和24小时收集细胞（每个实验条件设置3个孔重复），提取总RNA并通过微阵列（microarray）技术开展分析。将同一实验条件下的重复孔总RNA按等量混合以制备每张阵列所需样本；每个处理组与对照组在每个时间点对应1张阵列。