RNA sequencing facilitates quantitative analysis of 28ζ CAR, BBζ CAR and aTCR T cell transcriptomes

Purpose: Analyzing the cytokine transcription and associated signalings of human 28ζ CAR, BBζ CAR and aTCR T cells Methods: Human 28ζ CAR, BBζ CAR and aTCR T cells were stimulated with antigen peptides for four hours Results: Using an optimized data analysis workflow, we mapped about 50 million sequence reads per sample to the human genome (build GRCh38). The mRNA profiles of human 28ζ CAR, BBζ CAR and aTCR T cells upon stimulation with antigen peptides for four hours. There are two sample replicates for the human 28ζ CAR, BBζ CAR and aTCR T cells.

研究目的：分析人源28ζ嵌合抗原受体（Chimeric Antigen Receptor, CAR）、BBζ CAR以及aTCR T细胞的细胞因子转录特征及其相关信号通路。实验方法：将人源28ζ CAR、BBζ CAR以及aTCR T细胞以抗原肽刺激4小时。实验结果：采用优化后的数据分析流程，我们将每个样本的约5000万条测序读段比对至人类参考基因组GRCh38版本。本数据集包含经抗原肽刺激4小时后人源28ζ CAR、BBζ CAR以及aTCR T细胞的mRNA表达谱。上述三类T细胞样本均设置了两个生物学重复。