Determine the genome-wide localization of nmad-1 and top-2 in the transgenic flag tagged strains

We report the chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) on flag tagged top-2 and nmad-1 transgeneic strains. We generated over 200 million reads from the IP'd DNA as well as input controls. We found that nmad-1 cobound with top-2 for approximately 80% of regions with p < 0.0001 (Fisher's exact test). Top-2 localization was also depedent on nmad-1 as half of the sites were no longer bound by top2 after nmad-1 deletion. Overall design: Examine the nmad-1 and top-2 in wild type C.elegans thorughout the genome

本研究针对携带FLAG标签的top-2与nmad-1转基因品系，开展了染色质免疫共沉淀联合下一代测序（chromatin immunoprecipitation followed by next generation sequencing，ChIP-seq）实验。我们从免疫沉淀所得DNA及输入对照样本中，共获得超过2亿条测序读段（reads）。分析结果显示，在p值小于0.0001（费舍尔精确检验，Fisher's exact test）的区域中，约80%的区域存在nmad-1与top-2共同结合的现象。top-2的基因组定位同样依赖nmad-1：在nmad-1基因敲除后，有一半的结合位点不再被top-2识别结合。实验整体设计：在全基因组层面检测野生型秀丽隐杆线虫（Caenorhabditis elegans，C. elegans）中nmad-1与top-2的结合情况。