Expression data from preadipocytes isolated from laying hen II

Adipogenesis is one of the most intensively studied models of cellular differentiation, and transcriptional activities involved in the process have been extensively investigated. We analyzed transcripts differentially expressed between hen preadipocytes treated with adipogenic cocktail containing 500 nM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, 20 µg/mL insulin and 300 μM OA (DMIOA) and non-treated control cells and transcripts differentially expressed between preadipocytes treated with DMIOA alone and those treated with a combination of DMIOA and 20(S)-hydroxycholesterol (DMIOA + 20(S)) using Affymetrix GeneChip® Chicken Genome Array containing 28,000 transcripts. Preadipocytes were isolated from hen abdominal tissue, cultured and treated with DMIOA, DMIOA+Retinoic Acid

脂肪生成（Adipogenesis）是当前研究最为深入的细胞分化模型之一，该进程所涉及的转录调控活性已得到广泛探究。本研究利用搭载28000个转录本的Affymetrix GeneChip® 鸡全基因组芯片，开展两组差异表达转录本的分析：其一为经成脂诱导混合液（含500 nM地塞米松、0.5 mM 3-异丁基-1-甲基黄嘌呤、20 μg/mL胰岛素及300 μM油酸，该混合液缩写为DMIOA）处理的鸡前体脂肪细胞，与未处理的对照细胞之间的差异转录本；其二为仅用DMIOA处理的前体脂肪细胞，与经DMIOA联合20(S)-羟基胆固醇（20(S)-hydroxycholesterol，即DMIOA + 20(S)）处理的前体脂肪细胞之间的差异转录本。本研究中的鸡前体脂肪细胞分离自鸡腹部脂肪组织，经体外培养后分别采用DMIOA、DMIOA+视黄酸（Retinoic Acid）进行处理。