Sequencing marine seafloor samples

While marine seafloor covers 70 % of Earth's surface, the literature currently lacks comprehensive benchmarking of sequence variant resolution analytic tools used for metabarcoding high-diversity environmental samples. In this study, sequence variant tools (Dada2, Deblur, Swarm and UNOISE) were validated, using 30 sediment samples with three technical replicates each. Five common DNA extraction kits were used (Magpure soil, Magpure stool, Powersoil KF, Powersoil pro and Zymo Research QuickDNA), and a total of 450 DNA samples were sequenced using Illumina MiSeq.

尽管海洋海底覆盖了地球70%的地表面积，但当前学界仍缺乏针对高多样性环境样本元条形码（metabarcoding）分析所用序列变异解析工具的全面基准评测。本研究针对四款序列变异解析工具（Dada2、Deblur、Swarm及UNOISE）开展验证实验，实验材料为30份沉积物样本，每份设置三次技术重复。本次研究共使用五种常用DNA提取试剂盒，分别为Magpure soil、Magpure stool、Powersoil KF、Powersoil pro及Zymo Research QuickDNA，最终共计450份DNA样本通过Illumina MiSeq测序平台完成测序。