five

Table 2 in Two new species of Ophiostomatales (Sordariomycetes) associated with the bark beetle Dryocoetes alni from Poland

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Table 2. Information on PCR primers used in this study. LocusPrimersFungiITS1-5.8SITS1-F (Gardes and Bruns 1993), ITS4 (White et al. 1990)Ceratocystiopsis, Leptographium28SLR0R, LR5 (Vilgalys and Hester 1990)CeratocystiopsisITS2-28SITS3 (White et al. 1990), LR3 (Vilgalys and Hester 1990)LeptographiumTUB2Bt2a, Bt2b (Glass and Donaldson 1995)CeratocystiopsisT10 (O’Donnell and Cigelnik 1997), Bt2b (Glass and Donaldson 1995)LeptographiumACTLepact-F, Lepact-R (Lim et al. 2004)LeptographiumCALCL3F, CL3R (De Beer et al. 2016)LeptographiumTEF1-αF-728F (Carbone and Kohn 1999), EF2 (O’Donnell et al. 1998)CeratocystiopsisEF1F, EF2R (Jacobs et al. 2004)Leptographium

表2 本研究中使用的聚合酶链式反应(PCR)引物信息如下:1. 针对真菌的内部转录间隔区1-5.8S(ITS1-5.8S)区域,所用引物为ITS1-F(引自Gardes与Bruns,1993)、ITS4(引自White等,1990);2. 针对长喙壳孢属(Ceratocystiopsis)与细顶孢属(Leptographium)的28S核糖体RNA(28S rRNA)区域,所用引物为LR0R、LR5(引自Vilgalys与Hester,1990);3. 针对长喙壳孢属(Ceratocystiopsis)的ITS2-28S区域,所用引物为ITS3(引自White等,1990)、LR3(引自Vilgalys与Hester,1990);4. 针对细顶孢属(Leptographium)的β微管蛋白2基因(TUB2),所用引物为Bt2a、Bt2b(引自Glass与Donaldson,1995);5. 针对长喙壳孢属(Ceratocystiopsis)的TUB2基因,所用引物为T10(引自O’Donnell与Cigelnik,1997)、Bt2b(引自Glass与Donaldson,1995);6. 针对细顶孢属(Leptographium)的肌动蛋白基因(ACT),所用引物为Lepact-F、Lepact-R(引自Lim等,2004);7. 针对细顶孢属(Leptographium)的钙调蛋白基因(CAL),所用引物为CL3F、CL3R(引自De Beer等,2016);8. 针对细顶孢属(Leptographium)的翻译延伸因子1-α(TEF1-α)基因,所用引物为F-728F(引自Carbone与Kohn,1999)、EF2(引自O’Donnell等,1998);9. 针对长喙壳孢属(Ceratocystiopsis)的TEF1-α基因,所用引物为EF1F、EF2R(引自Jacobs等,2004)
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2025-04-04
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