Fish sequences DNA barcode (DP1.20105.001)

This data product contains the quality-controlled laboratory metadata and QA results for NEON's cytochrome oxidase I (COI) barcoding of fish sequences. Fin clips are taken from a subset of collected fish for DNA analysis. The DNA barcoding procedure involves the removal of tissue, extracting and sequencing DNA from the tissue, and matching that sequence data to sequences from previously identified voucher specimens. DNA analysis serves a number of purposes, including verification of taxonomy of specimens that do not receive expert identification, clarification of the taxonomy of rare or cryptic species, and characterization of diversity using molecular markers. For additional details, see the user guide, protocols, and science design listed in the Documentation section in this data product's details webpage. Queries for this data product will return metadata tables formatted for submission to the Barcode of Life Database. These queries will also provide links to the actual sequence data, which are publicly available on the Barcode of Life Datasystem (BOLD, http://www.barcodinglife.com/). The sequence data can be obtained by following the links from the NEON data portal, or by directly querying NEON data sets on the BOLD server. From the NEON portal, the link "BOLD Project: Fish sequences DNA barcode" redirects to a page on the BOLD public data portal for the queried data. This is a dynamic link and will automatically update based on the user query.

Latency:
The expected time from data and/or sample collection in the field to data publication is as follows, for each of the data tables (in days) in the downloaded data package. See the Data Product User Guide for more information.

fsh_BOLDcollectionData: 390

fsh_BOLDspecimenDetails: 390

fsh_BOLDtaxonomy: 390

fsh_BOLDvoucherInfo: 390

本数据产品包含经过质量管控的实验室元数据，以及美国国家生态观测站网络（NEON, National Ecological Observatory Network）针对鱼类细胞色素氧化酶I（COI, cytochrome oxidase I）条形码测序的质量保证（QA, Quality Assurance）结果。研究人员从已采集的鱼类样本中选取子集，剪取其鳍组织用于DNA分析。DNA条形码流程包括组织取样、提取并测序组织中的DNA，再将所得序列与此前已完成鉴定的凭证标本序列进行比对匹配。DNA分析具备多重用途，包括验证未经过专家鉴定的样本的分类学信息、厘清稀有或隐存物种的分类学关系，以及利用分子标记表征物种多样性。如需了解更多细节，请参阅本数据产品详情网页中“文档”板块所列的用户指南、实验方案及科学设计说明。

针对本数据产品的查询将返回可提交至生命条形码数据库（Barcode of Life Database）的元数据表，同时还将提供指向实际序列数据的链接。此类序列数据可在生命条形码数据系统（BOLD, Barcode of Life Datasystem, http://www.barcodinglife.com/）公开获取。序列数据可通过国家生态观测站网络数据门户中的对应链接获取，或直接在BOLD服务器上查询国家生态观测站网络的数据集。从国家生态观测站网络门户进入时，链接"BOLD Project: Fish sequences DNA barcode"将重定向至BOLD公开数据门户中对应查询数据的页面；该链接为动态链接，将根据用户的查询自动更新。

数据延迟说明：
针对下载数据包中的各数据表格，从野外数据和/或样本采集到数据公开的预期耗时（单位：天）如下，详见《数据产品用户指南》以获取更多信息。
fsh_BOLDcollectionData：390
fsh_BOLDspecimenDetails：390
fsh_BOLDtaxonomy：390
fsh_BOLDvoucherInfo：390