Additional file 2 of Vanillic acid and methoxyhydroquinone production from guaiacyl units and related aromatic compounds using Aspergillus niger cell factories

Additional file 2: Table S1. Molar and mass yield on vanillin, vanillic acid or methoxyhydroquinone production from 5 mmol/L on guaiacyl units and related aromatic compounds obtained by ∆vdhA, ∆vhyA and ∆mhdA. Mean values and standard deviations of three biological replicates. Table S2. Transcriptome data of hqdA and NRRL3_4956. Fold change (log2) compared to a no carbon source control and p-values were calculated with DESeq2. Table S3. Primers used in this study. In red are the regions overlapping pyrG. Table S4. Fungal genomes used for the phylogenetic study of VdhA and MhdA. Genomes were obtained from JGI MycoCosm ( https://mycocosm.jgi.doe.gov/mycocosm/home ). Table S5. Amino acid sequences used for the phylogenetic analysis of MhdA and VdhA that were curated manually or with the gene prediction software Augustus.附加文件2：表S1。以5 mmol/L愈创木基单元（guaiacyl units）及相关芳香族化合物为底物，由∆vdhA、∆vhyA和∆mhdA菌株生产香兰素、香兰酸或甲氧基对苯二酚时的摩尔产率与质量产率。结果为三次生物学重复的平均值与标准差。表S2：hqdA与NRRL3_4956的转录组数据。以无碳源对照组为参照的倍数变化（log2），其P值通过DESeq2软件计算得到。表S3：本研究使用的引物。与pyrG存在重叠的区域以红色标注。表S4：用于VdhA与MhdA系统发育研究的真菌基因组。基因组数据获取自JGI MycoCosm数据库（https://mycocosm.jgi.doe.gov/mycocosm/home）。表S5：用于MhdA与VdhA系统发育分析的氨基酸序列，这些序列经人工整理或通过基因预测软件Augustus预测得到。