Transcriptomics and chromatin accessibility analysis of MLL-AF9 early responses in mouse bone marrow cells. Transcriptomics and chromatin accessibility analysis of MLL-AF9 early responses in mouse bone marrow cells

We investigated early changes of gene expressions and chromatin accessibilities in hematopoietic stem and progenitor cells (HSPCs) in response to MLL-AF9 oncogene expressions. Using RNA-Seq and ATAC-Seq, we analyzed HSPCs briefly exposed to MLL-AF9 for one or two days. Such analysis addressed the initial changes during malignant transformation, and provided insights into the primary functions of MLL-AF9 oncogene. Overall design: Early cellular responses to MLL-AF9 oncogene expression were determined using RNA-Seq and ATAC-Seq of HSPCs exposed to MLL-AF9 for one or two days. Freshly isolated HSPCs and HSPCs cultured without MLL-AF9 expression were used as controls, in duplicates.

本研究探究了造血干细胞和祖细胞（hematopoietic stem and progenitor cells，HSPCs）在响应MLL-AF9癌基因表达时的基因表达与染色质可及性早期变化。本研究通过RNA测序（RNA-Seq）与转座酶可及性测序（ATAC-Seq），对仅短暂暴露于MLL-AF9癌基因1至2天的HSPCs开展分析。该分析旨在阐明恶性转化过程中的初始分子变化，并为解析MLL-AF9癌基因的核心功能提供理论见解。
整体实验设计：本研究通过对暴露于MLL-AF9癌基因1至2天的HSPCs进行RNA-Seq与ATAC-Seq分析，明确其对MLL-AF9癌基因表达的早期细胞应答。实验以新鲜分离的HSPCs以及未表达MLL-AF9的培养HSPCs作为对照组，每组设置2个生物学重复。