Chemical translatome analysis in AML cells treated with different drug combinations

Our prior research has shown that AML with FLT3-ITD is particularly susceptible to the combination of FLT3 inhibitors and protein synthesis inhibitors when compared to FLT3-WT. In this study, we employed a click chemistry-based approach to quantify the alterations in the translatome under FLT3 inhibition and protein synthesis inhibition in AML cells.

我们既往的研究表明，与野生型FLT3（FLT3-WT）急性髓系白血病（Acute Myeloid Leukemia，AML）细胞相比，携带FLT3内部串联重复（FLT3-ITD）的AML细胞对FLT3抑制剂与蛋白质合成抑制剂的联合给药方案尤为敏感。本研究采用基于点击化学（click chemistry）的实验方法，对AML细胞在FLT3抑制及蛋白质合成抑制条件下的翻译组（translatome）变化进行了定量分析。