Cardiotrophin Like Cytokine Factor 1 (CLCF1) alleviates bone loss and osteoclastogenesis in osteoporosis mouse models through activating interferon signaling and repressing the nuclear factor-κB signaling pathway. Cardiotrophin Like Cytokine Factor 1 (CLCF1) alleviates bone loss and osteoclastogenesis in osteoporosis mouse models through activating interferon signaling and repressing the nuclear factor-κB signaling pathway

We explored the functional role of CLCF1 in osteoclastogenesis and bone loss associated with osteoporosis. Surprisingly, the administration of recombinant CLCF1 repressed excessive bone loss in ovariectomized mice and reduced the levels of local osteolytic lesions induced by a RANKL injection. Likewise, the addition of recombinant CLCF1 to RANKL-stimulated monocytes resulted in a significant suppression in the number of differentiated osteoclasts and their resorption activity on dentine slices. Overall design: RNA-seq for macrophages stimulated by macrophage colony-stimulating factor, receptor activator of nuclear factor kappa-b ligand and CLCF1

本研究探讨了心肌营养素样细胞因子1（CLCF1）在骨质疏松相关破骨细胞生成（osteoclastogenesis）与骨丢失中的功能作用。令人意外的是，向去卵巢小鼠体内注射重组CLCF1，可抑制其过度骨丢失，并减轻核因子κB受体活化因子配体（Receptor Activator of Nuclear Factor kappa-B Ligand，RANKL）注射诱导的局部溶骨性病变程度。同样，在经RANKL刺激的单核细胞中加入重组CLCF1，可显著抑制分化成熟的破骨细胞数量及其在牙本质切片上的骨吸收活性。实验整体设计：对经巨噬细胞集落刺激因子（macrophage colony-stimulating factor）、核因子κB受体活化因子配体（RANKL）以及CLCF1刺激的巨噬细胞开展RNA测序（RNA-seq）。