RNA sequencingof M. tuberculosis H37Rv WT strain, cysE mutant strain and mshD mutant strain

We next asked whether any transcriptional response was associated with the redox, metabolic, and bioenergetic changes we described in cysE mutant strain and mshD mutant strain . RNA-seq analysis was carried out to analyze the mRNA levels of WT , cysE mutant strain and mshD mutant strain under normal cultivation conditions.

接下来，我们旨在探究是否存在与我们此前在cysE突变菌株（cysE mutant strain）和mshD突变菌株（mshD mutant strain）中报道的氧化还原、代谢及生物能学变化相关的转录应答。我们开展了RNA测序（RNA-seq）分析，以检测正常培养条件下野生型（Wild Type，WT）、cysE突变菌株及mshD突变菌株的mRNA表达水平。