CIL:912, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:911.

将家兔腰大肌骨骼肌纤维固定于恒定长度，并通过浸入甘油诱导其进入肌肉僵直状态。随后将纤维置于Heuser型低温压榨仪中，与液氦冷却的铜块接触以实现快速冷冻；接着将样品放入Balzers 400型冷冻断裂仪内进行冷冻蚀刻，以铂-碳（Pt-C）在24度角度下完成旋转复型，最后在加速电压为100千电子伏的JEOL 100CX2型透射电子显微镜下进行观测。
图像以75000倍的放大倍率记录于柯达4489胶片上，采集了±5度倾角的立体图像对。随后使用尼康Coolscan 9000ED扫描仪，以20微米的像素间距对胶片进行扫描。
本立体图像对对应CIL:911。