Multigenerational effects of antibiotic exposure on Daphnia magna

In this study, we examined the impacts of chronic antibiotic exposure across five generations of the keystone zooplankton species Daphnia magna. We utilized an antibiotic suite of aztreonam, sulfamethoxazole, and erythromycin, using sublethal doses in each generation. At each generation, we used a split-brood design, moving 24 juveniles to a no antibiotic treatment and 24 into antibiotics to understand how the microbiome recovered after more generations of exposure.

本研究针对关键浮游动物物种大型溞（Daphnia magna）的五代种群，探究了长期抗生素暴露的影响效应。本研究采用氨曲南（aztreonam）、磺胺甲恶唑（sulfamethoxazole）与红霉素（erythromycin）组成的抗生素组合，在每一代实验中均使用亚致死剂量进行暴露处理。每一代实验均采用同胞分割设计（split-brood design）：将24只幼体转移至无抗生素处理组，另外24只幼体分配至抗生素暴露组，以解析多代抗生素暴露后宿主微生物组（microbiome）的恢复模式。