Regulation of Chromatin Looping by CTCF and Cohesin

As part of a study to understand the dynamics of chromatin looping and its regulation by CTCF and cohesin, we have determined the genome-wide binding profiles of CTCF and cohesin (Smc1a subunit) in a genome-edited mouse Embryonic Stem Cell (mESC) line referred to as clone C65. We have also performed 3D genome mapping using Micro-C in another genome-edited mESC line referred to as clone C36. We performed Micro-C in the mESC clone C36 (5 replicates) and ChIP-seq in the mESC clone C65 (input, normal rabbit IgG, rabbit anti-CTCF and rabbit anti-Smc1a antibodies, single replicates).

为解析染色质环（chromatin looping）的动态变化及其受CCCTC结合因子（CTCF）与黏连蛋白（cohesin）的调控机制，我们在一株命名为克隆C65的基因组编辑小鼠胚胎干细胞（mouse Embryonic Stem Cell, mESC）系中，测定了CTCF与黏连蛋白（cohesin）的Smc1a亚基（Smc1a subunit）的全基因组结合谱。此外，我们在另一株命名为克隆C36的基因组编辑mESC系中，采用微C（Micro-C）技术完成了三维基因组图谱绘制。本研究对mESC克隆C36进行了Micro-C测序（共5次生物学重复），并对mESC克隆C65开展了染色质免疫共沉淀测序（ChIP-seq），所用对照及抗体包括Input对照、正常兔IgG、兔抗CTCF抗体与兔抗Smc1a抗体，均为单次生物学重复。