SIPA1L1/SPAR1 Interacts with the Neurabin Family of Proteins and is Involved in GPCR Signaling. Matsuura et al. Raw data for super-resolution colocalization analyses.

Super-resolution microscopic images stained for SIPA1L1 and its candidate interacting-proteins in the neuropil region of layer V cerebral cortex or hippocampal CA1 area were acquired using Olympus SD-OSR, a confocal-based spinning disk super-resolution microscope, which implements structured illumination microscopy (SIM). Four serial Z-stack images (200-nm step size) of 1024 x 1024 pixels (41 x 41 um2)/image were used for colocalization analyses. w1 files are for SIPA1L1 and w2 files for others in all folders with the exception of drebrin and actinin where SIPA1L1 is w2.

本数据集通过搭载结构化照明显微镜（structured illumination microscopy，SIM）技术的奥林巴斯SD-OSR共聚焦转盘式超分辨显微镜采集得到，成像样本为大脑皮层第五层神经毡区域或海马CA1区中经SIPA1L1及其候选互作蛋白染色的组织。本次实验使用四张连续Z轴堆叠图像开展共定位分析，单张图像分辨率为1024×1024像素，对应视野面积41×41μm²，Z轴步长为200nm。在所有文件夹中，w1格式文件对应SIPA1L1的成像数据，w2格式文件对应其余靶标蛋白的成像数据；但在德布林蛋白（drebrin）与辅肌动蛋白（actinin）对应的文件夹中，SIPA1L1的成像数据采用w2格式文件。