The effect of epigenetics on diapause of Asian corn borer larvae [ChIP-seq]

H3K9me3 and H3K27me3 were involved in diapause regulation of O. furnacalis larvae The newly molted fourth instar larvae were collected and maintained at 20 °C under a photoperiod of 12L: 12D for at least 30 days to achieve diapause (Guo et al, 2013). The newly molted fourth instar larvae maintained for 20 days at a photoperiod of 20 ° C and 12L: 12D were in a pre-diapause state. CHIP-seq was performed on the heads of diapause, pre-diapause and non-diapause Asian corn borer larvae using antibodies H3K9me3 and H3K27me3 .

组蛋白H3赖氨酸9三甲基化（H3K9me3）与组蛋白H3赖氨酸27三甲基化（H3K27me3）参与了亚洲玉米螟（Ostrinia furnacalis）幼虫的滞育（diapause）调控。本研究收集刚蜕皮的四龄幼虫，将其置于20℃、光周期12L:12D的条件下饲养至少30天，以诱导其进入滞育状态（Guo等，2013）。将在20℃、光周期12L:12D条件下饲养20天的刚蜕皮四龄幼虫划定为预滞育状态个体。分别以H3K9me3和H3K27me3抗体，对滞育、预滞育及非滞育亚洲玉米螟幼虫的头部组织开展染色质免疫共沉淀测序（CHIP-seq）。