Identification of genome editing nucleases off-target activity

ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editingITR-Seq作为一种下一代测序（next-generation sequencing）检测方法，可在腺相关病毒载体介导的基因组编辑完成后，于体内鉴定全基因组范围内的DNA编辑位点。