Determination of Shavenbaby target genes in mature Enterocytes (mECs)

Shavenbaby (Svb) transcription factor is involved in the homeostasis of intestinal stem cells (ISCs), and the differentiation of their enteroblast progeny (EB) into enterocytes (EC). Svb is produced as a long repressor protein called SvbRepressor (SvbREP). In presence of Pri peptides SvbREP is processed into a shorter activator form (SvbACT) through partial proteasomal degradation of the REP domain. In ISCs/EBs, Pri triggers SvbREP to ACT maturation, while SvbREP form accumulates in differentiated enterocytes. The absence of Svb promote the apoptosis of the intestinal stem cells. In ISCs/EBs, the ectopic expression of SvbREP form promotes the differentiation and the overexpression of SvbACT promotes hyperplasia. (Al Hayek, et al 2020) Based on these functional analyses, Svb appears to control ISC/EB survival and behavior, balancing proliferation and differentiation through a molecular switch between its 2 antagonistic isoforms. In this study, we determine the Svb target genes in different cell types along the intestinal cell lineage. This particular metadata sheet refers to early enterocytes (mEC) transcriptomic data (RNAseq) in control condition and upon modulation of Svb function. Overall design: The samples correspond to control (mEC Ctrl), Svb-knock down ECs (mEC RNAiSvb), SvbACT constitutive form overexpression (mEC SvbAct). Three biological replicates were performed for each genotype (except for the Ctrl n=2), on different calendar days.

Shavenbaby（Svb）转录因子参与肠干细胞（intestinal stem cells，ISCs）的稳态维持，并调控其成肠母细胞后代（enteroblast progeny，EB）分化为肠上皮细胞（enterocytes，EC）的过程。Svb以名为Svb阻遏蛋白（SvbRepressor，SvbREP）的长链阻遏蛋白形式合成。当存在Pri肽（Pri peptides）时，SvbREP会通过REP结构域的部分蛋白酶体降解，被加工为较短的激活型形式（SvbACT）。在肠干细胞/成肠母细胞（ISCs/EBs）中，Pri可触发SvbREP向SvbACT的成熟转化，而SvbREP形式会在已分化的肠上皮细胞中积累。Svb缺失会诱导肠干细胞发生凋亡。在肠干细胞/成肠母细胞中，异位表达SvbREP会促进细胞分化，而过表达SvbACT则会引发细胞增生（Al Hayek等人，2020）。基于上述功能分析，Svb似乎通过其两种拮抗亚型间的分子开关，调控肠干细胞/成肠母细胞的存活与行为，平衡细胞增殖与分化过程。本研究旨在明确肠细胞谱系不同细胞类型中Svb的靶基因。本元数据表格对应对照条件下以及调控Svb功能后的早期肠上皮细胞（early enterocytes，mEC）转录组数据（RNA测序，RNAseq）。实验整体设计如下：样本分为对照组（mEC Ctrl）、Svb敲低肠上皮细胞（mEC RNAiSvb）、组成型过表达SvbACT的肠上皮细胞（mEC SvbAct）。除对照组样本量n=2外，每种基因型均设置3次生物学重复，实验于不同日历日期完成。