RNAseq of SMAD2/3DKO and Smad4KO in pluripotent mESC and EBs

To dissected the roles of SMAD2/3 and SMAD4 in mouse embryonic stem cells (mESC), knockouts of SMAD2/3 (SMAD2 and SMAD3 double knockout, S2/3DKO) and SMAD4 (SMAD4 knockout, S4KO) were used to conduct RNAseq in ES and EB conditions. Overall design: WT, S2/3DKO and S4KO cells were collected at ES (D0) and embryoid body (EB) Day 4 conditions. Each sample were duplicated for RNAseq analysis.

为解析SMAD2/3与SMAD4在小鼠胚胎干细胞（mouse embryonic stem cells, mESC）中的调控作用，研究人员构建了SMAD2/3双敲除模型（SMAD2与SMAD3双敲除，S2/3DKO）与SMAD4单敲除模型（SMAD4敲除，S4KO），并分别在胚胎干细胞（ES）及拟胚体（EB）培养条件下开展RNA测序（RNAseq）分析。总体实验设计：分别在胚胎干细胞培养状态（第0天，D0）与拟胚体培养第4天（EB Day 4）的条件下，收集野生型（wild type, WT）、S2/3DKO及S4KO细胞样本，每个样本均设置2次生物学重复用于RNAseq分析。