ExtFig9A_RACE_PR8-NS_R1-R2-R3_LG290-292-293_annotated.tif

Xrn1 ko A549 cells were infected with WT or PR8-PA(∆X), or mock infected. RNA was extracted to run 5’ RACE using primers ~150-200 nt downstream of GCUG sites that are predicted to be inside a hairpin loop within viral mRNAs (in segment 4 or 8 as indicated)

将Xrn1敲除的A549细胞分别以野生型（WT）、PR8-PA(∆X)毒株感染，同时设置空白感染对照。提取细胞RNA后，使用位于GCUG位点下游约150~200 nt的引物开展5'末端快速扩增（5' RACE）实验；上述GCUG位点被预测位于病毒mRNA的发夹环结构内，相关位点对应第4或第8个病毒基因节段（如标注所示）。