Expression data from Aracidopsis roots

Sulfate is recognized as a primary sulfur source for plant growth and effects of thiosulfate as a sulfur source on plant growth and metabolism, gene expression have not been studied. We used microarrays to detail the global programme of gene expression in response to thiosulfate as a sulfur source. Arabidopsis Col-0 WT plants were hydroponically grown for 8 wks under short day condition, and roots were sampled for RNA extraction and hybridization on Affymetrix microarrays.

硫酸盐（Sulfate）被公认为植物生长的主要硫源，而以硫代硫酸盐（thiosulfate）作为硫源时，其对植物生长、代谢及基因表达的影响尚未得到研究。本研究利用基因芯片（microarrays）详细解析了植物响应硫代硫酸盐作为硫源时的全局基因表达谱。实验中，我们将拟南芥（Arabidopsis）Col-0野生型植株置于短日照条件下水培培养8周，随后采集根部组织进行RNA提取，并在Affymetrix基因芯片上完成杂交实验。