Expression data from Triticum aestivum L. roots

To better understand the regulatory mechanisms of water stress response in wheat, the transcript profiles in roots of two wheat genotypes, namely, drought tolerant 'Luohan No.2' (LH) and drought susceptible 'Chinese Spring' (CS) under water-stress were comparatively analyzed by using the Affymetrix wheat GeneChip®. A total of 3831 transcripts displayed 2-fold or more expression changes, 1593 transcripts were induced compared with 2238 transcripts were repressed, in LH under water-stress; Relatively fewer transcripts were drought responsive in CS, 1404 transcripts were induced and 1493 were repressed. Comparatively, 569 transcripts were commonly induced and 424 transcripts commonly repressed in LH and CS under water-stress. 689 transcripts (757 probe sets) identified from LH and 537 transcripts (575 probe sets) from CS were annotated and classified into 10 functional categories, and 74 transcripts derived from 80 probe sets displayed the change ratios no less than 16 in LH or CS. Several kinds of candidate genes were differentially expressed between the LH and CS, which could be responsible for the difference in drought tolerance of the two genotypes. Two common wheat (Triticum aestivum L.) cultivars, Luohan No.2 (LH) and Chinese Spring (CS), were used for this study. Seedlings at the two leaf stage were stressed by cultured in PEG solutions for 6h, and some other seedlings were cultured in tap water as control. Root samples of LH and CS at 6h after the stress treatment and untreated control were prepared for microarray analysis.

为深入解析小麦水分胁迫响应的调控机制，本研究采用Affymetrix小麦基因芯片®，对两个小麦基因型——耐旱型品种‘洛旱2号’（Luohan No.2，简称LH）与干旱敏感型品种‘中国春’（Chinese Spring，简称CS）在水分胁迫条件下的根系转录本表达谱进行了比较分析。在水分胁迫下的LH中，共计3831个转录本的表达量变化幅度达2倍及以上，其中1593个转录本被诱导表达，2238个转录本被抑制表达；相对而言，CS中响应干旱的转录本数量更少，共1404个被诱导表达、1493个被抑制表达。比较而言，LH与CS在水分胁迫下共有569个转录本被共同诱导表达，424个转录本被共同抑制表达。从LH中鉴定得到的689个转录本（对应757个探针组）以及从CS中鉴定得到的537个转录本（对应575个探针组）均被注释并划分为10个功能类别；另有74个转录本（对应80个探针组）在LH或CS中的表达变化倍数不低于16倍。多个候选基因在LH与CS间存在差异表达，这可能是两个小麦基因型耐旱性差异的成因。本研究选用的两个普通小麦（Triticum aestivum L.）品种为洛旱2号（LH）与中国春（CS）。将处于两叶期的小麦幼苗置于聚乙二醇（PEG）溶液中进行6小时的水分胁迫处理，同时以部分置于自来水培养的幼苗作为对照。分别收集胁迫处理6小时后以及未处理对照组的LH与CS根系样品，用于芯片分析。