DataSheet1_Qualitative and quantitative chemical analysis of Leptadenia hastata: exploring a traditional african medicinal plant.docx

Leptadenia hastata (Pers.) Decne is a commonly used food source and prescribed as a traditional African medicine for treatment of various diseases, such as diabetes, skin disorders, wounds, and ulcers. However, quality control has become a bottleneck restricting the therapeutic development and utilization of this plant. In this study, a reliable method for qualitative and quantitative determination of components in Leptadenia hastata was established. The components of L. hastata were profiled using ultra-high performance liquid chromatography coupled with quadruple time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS). Subsequently, an ultra-high performance tandem diode array detector (UHPLC-DAD)-based method was used for simultaneous quantitative analysis of five major constituents in six batches of L. hastata samples. As a result, 35 compounds were tentatively identified. The quantities of the five constituents (vicenin-Ⅱ, orientin, schaftoside, chrysin 6-C-arabinoside 8-C-glucoside, chrysin 6-C-glucoside 8-C-arabinoside) were determined as 124.8–156.9 μg/g, 170.5–216.0 μg/g, 61.31–93.73 μg/g, 85.13–119.3 μg/g and 99.82–129.4 μg/g, respectively. This method offers a successful strategy for precise and effective evaluation of the constituents of L. hastata, providing a robust foundation for holistic quality assessment of medicinal plants.

戟叶鹅绒藤（Leptadenia hastata (Pers.) Decne）是一种常用食用资源，同时作为非洲传统药物被用于治疗糖尿病、皮肤疾病、创伤及溃疡等多种病症。然而，质量控制已成为制约该植物药用开发与利用的瓶颈。本研究建立了一种可靠的戟叶鹅绒藤成分定性定量分析方法：采用超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-Q-TOF-MS）对其成分进行谱图分析与表征；随后基于超高效液相色谱-二极管阵列检测器（UHPLC-DAD）的分析方法，对6批戟叶鹅绒藤样品中的5种主要成分开展同时定量检测。实验结果表明，共初步鉴定出35种化合物。经测定，这5种成分（vicenin-Ⅱ、orientin、schaftoside、chrysin 6-C-arabinoside 8-C-glucoside、chrysin 6-C-glucoside 8-C-arabinoside）的含量分别为124.8~156.9 μg/g、170.5~216.0 μg/g、61.31~93.73 μg/g、85.13~119.3 μg/g及99.82~129.4 μg/g。该方法为戟叶鹅绒藤的成分精准有效评价提供了可行策略，可为药用植物的整体质量评价奠定坚实基础。