Analysis of gene expression in sublines of DU145 human prostate cancer cells created through in vivo metastatic selection

DU145 human prostate cancer cells were injected into the prostate of nude mice and cells metastasizing to lymph node (LN1) grown and experiment repeated to make LN2-4. DU145 human prostate cancer cells were injected into the tail vein of nude mice and cells metastasizing to lung (ivLU1) grown and experiment repeated to make ivLU2-4. 8 groups representing individual cell lines

将DU145人前列腺癌细胞接种至裸鼠（nude mice）前列腺内，分离获取转移至淋巴结（lymph node，缩写LN）的癌细胞，经体外培养扩增后命名为LN1细胞亚系，重复上述实验流程以构建LN2至LN4系列细胞亚系。将DU145人前列腺癌细胞经尾静脉注射至裸鼠体内，分离获取转移至肺组织的癌细胞，经体外培养扩增后命名为ivLU1细胞亚系，重复上述实验流程以构建ivLU2至ivLU4系列细胞亚系。本数据集共包含8组独立的细胞系。