CIL:34928

Localization of cross-linking proteins in fibroblast cytoskeleton. mmuno-EM of the cell interior or cell edge (CIL 34925) of cytochalasin D-treated Xenopus fibroblasts stained with p21 (Arp2/3) primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal Arp2/3 complex at Y-junctions at cell edge. Fluorescence microscopy and corresponding intensity profiles double stained with TRITC-phalloidin (red) and p21 antibodies (green) is available at CIL 34922. Image corresponds to Figure 5g from J Cell Biol. 1999 May 31;145(5):1009-26.成纤维细胞骨架中交联蛋白的定位研究：对经细胞松弛素D处理的爪蟾成纤维细胞的细胞内部或细胞边缘（CIL 34925）进行免疫电子显微镜（Immuno-EM）成像，样品采用p21（Arp2/3）一抗与10 nm金标二抗进行染色。黄色金颗粒显示，Arp2/3复合物定位于细胞边缘的Y型连接位点。采用TRITC-鬼笔环肽（红色）与p21抗体（绿色）双重染色的荧光显微镜图像及对应强度曲线，可在CIL 34922获取。本图像对应《Journal of Cell Biology》1999年5月31日刊第145卷第5期第1009-1026页的图5g。