The m6A Reader IGF2BP2 Regulates Glutamine Metabolism and Represents a Therapeutic Target in Acute Myeloid Leukemia [Ribo-seq]

We report that m6A reader IGF2BP2 participates in the regulation of glutamine metabolism in AML. To further clarify whether IGF2BP2 knockdown (KD) leads to translational inhibition, we perform Ribo-seq in control and IGF2BP2 KD cells. MM6 cells infected with shNS or shIGF2BP2 (including shBP2B and shBP2D) were employed for ribo-seq.

本研究报道，m6A阅读蛋白（m6A reader）IGF2BP2参与调控急性髓系白血病（AML）中的谷氨酰胺代谢。为进一步明确IGF2BP2敲低（KD）是否会引发翻译抑制，我们对阴性对照及IGF2BP2敲低细胞开展了核糖体测序（Ribo-seq）实验。本研究采用感染了阴性对照短发夹RNA（shNS）或靶向IGF2BP2的短发夹RNA（包括shBP2B与shBP2D）的MM6细胞进行核糖体测序。