Next Generation Sequencing Facilitates Quantitative Analysis of NP1 and NP1/CRTCHA Gut Transcriptomes. Next Generation Sequencing Facilitates Quantitative Analysis of NP1 and NP1/CRTCHA Gut Transcriptomes

Differential expression analysis used the DESeq2 Bioconductor package, a model based on the negative binomial distribution. the estimates of dispersion and logarithmic fold changes incorporate data-driven prior distributions, Padj of genes were setted <0.05 to detect differential expressed ones.Our study represents the detailed analysis of of NP1 and NP1/CRTCHA drosophila gut transcriptomes, with biologic replicates, generated by RNA-seq technology.Transcriptome analysis indicated that the genes involved in proteasome assembly, ROS scavengers, and protein folding were highly enriched among those differentially expressed genes (DEGs) in CRTC overexpressing (CRTCOE) intestines. Overall design: Gut mRNA profiles of 3-5-day old NP1/+ and NP1/+;UASCRTCHA/+ drosophila

本研究采用基于负二项分布（negative binomial distribution）模型的DESeq2 生物信息学软件包（Bioconductor）开展差异表达分析。该分析的离散度估计值与对数倍变化值纳入了数据驱动的先验分布（prior distributions）；以基因校正后P值（Padj）<0.05作为筛选阈值，用于鉴定差异表达基因（differentially expressed genes, DEGs）。本研究通过RNA测序（RNA-seq）技术构建带有生物学重复（biological replicates）的样本，对NP1与NP1/CRTCHA果蝇（drosophila）的肠道转录组开展详细分析。转录组分析结果显示，在CRTC过表达（CRTC overexpressing, CRTCOE）果蝇肠道的差异表达基因中，参与蛋白酶体组装（proteasome assembly）、活性氧清除剂（ROS scavengers）以及蛋白质折叠（protein folding）的基因显著富集。实验设计概况：3-5日龄NP1/+与NP1/+;UASCRTCHA/+果蝇的肠道mRNA表达谱。