Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region"

<b>Raw landmark coordinates</b>Each trunk vertebrae (T1 to T13) per species obtained from landmarking 3D surface models of the specimens used in this study can be found in the zip file "Raw landmark coordinates.rar". Each file is labelled as follows: Vertebrae-"Species"-"Collection label"-"Vertebrae number". For example "Vertebrae-Ampelensis-OZ-62-G22595-T1". Collection labels can be found in Appendix table S1 and individual landmark descriptions in Appendix table S2.<br><b>Appendix table S1</b>Collection material analyzed and type of preservation: ethanol preserved and whole-mounts glycerine stored skeletons. Museum codes are: IBISS – University of Belgrade, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, RMNH.RENA and ZMA.RENA – Naturalis Biodiversity Center, Leiden, The Netherlands. N - sample size.<br><br><b>Appendix table S2</b>Brief anatomical descriptions of 27 landmarks configured to Lissotriton trunk vertebrae.<br><br><b>Appendix table S3</b>Frequency (%) of morphotypes over Lissotriton taxon / population and vertebra number.<br><b>Appendix table S4</b>Results on the phenotypic trajectory analysis with P-values for pairwise statistical assessment of a) the amount of species-specific shape changes along trunk region, i.e., trajectory size, b) the general orientation of shape changes in multivariate shape space, i.e., trajectory direction and c) the pattern of shape changes of vertebrae along the trunk region, i.e., trajectory shape.<br><b>Supplementary figure S1</b> Geographical distribution of the (sub-)species of the genus <i>Lissotriton</i> after Arntzen et al., 2009, Ianella et al., 2017 and Wielstra et al.,2018. Taxa are coded by colours as shown in the legend. Numbered dots indicate the localities of the populations that were studied, as also listed in Supplementary table S1. Note that no material was available for <i>L. italicus</i> and <i>L. lantzi.</i><i><br></i> <b>Supplementary figure S2</b> Phenotypic trajectories of shape changes along the trunk region of the newt genus <i>Lissotriton </i>expressed over the first (PC1) and third principal component axis (PC3). Trajectories were calculated for nine (sub-)species. The three populations of the <i>L. helveticus </i>were analysed separately<i>.</i> Taxa are coded by colours as shown in the legend. Connected dots represent the mean vertebrae (T) shape from T1 to T12, as is highlighted for <i>L.</i><i> helveticus</i> from Marcillé la Ville, France. Three-dimensional surface models visualize the corresponding shape changes.<br> <b>Supplementary figure S2_3D. </b> Three-dimensional graph showing phenotypic trajectories of shape changes along the trunk region in nine (sub-)species of the newt genus Lissotriton. Open the file with a web browser such as Google Chrome or Mozilla Firefox to visualize the trajectories in three dimensional morphospace that as defined by the first, second and third principal component axis (PC1, PC2 and PC3). <br>

<b>原始解剖地标坐标（Raw landmark coordinates）</b>
本研究所用标本的三维表面模型经地标标记后得到的每个物种的躯干椎骨（T1至T13）数据，均可在压缩包“Raw landmark coordinates.rar”中获取。各文件命名规则如下：Vertebrae-"物种名"-"馆藏编号"-"椎骨编号"，例如"Vertebrae-Ampelensis-OZ-62-G22595-T1"。馆藏编号详见附录表S1，各解剖地标点的详细说明详见附录表S2。
<br><b>附录表S1</b>
本次分析的馆藏标本及其保存方式：乙醇保存标本以及经甘油整体封存的骨骼标本。馆藏机构代码如下：IBISS – 塞尔维亚共和国国家研究院、贝尔格莱德大学生物研究所“斯尼沙·斯坦科维奇”（Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia）；RMNH.RENA与ZMA.RENA – 荷兰莱顿自然生物多样性中心（Naturalis Biodiversity Center, Leiden, The Netherlands）。N为样本量。
<br><br><b>附录表S2</b>
针对真螈属（Lissotriton）躯干椎骨设置的27个解剖地标点的简要说明。
<br><br><b>附录表S3</b>
基于真螈类群/种群及椎骨编号的形态类型出现频率（%）。
<br><b>附录表S4</b>
本附录呈现了表型轨迹分析的结果，包含用于三项成对统计评估的P值，具体涵盖：a）沿躯干区域的物种特异性形状变化幅度（即轨迹大小），b）多变量形状空间中形状变化的整体取向（即轨迹方向），以及c）沿躯干区域的椎骨形状变化模式（即轨迹形态）。
<br><b>补充图S1</b>
基于Arntzen等（2009）、Ianella等（2017）及Wielstra等（2018）的研究绘制的真螈属（Lissotriton）各（亚）种地理分布。类群按图例所示以颜色区分。带编号的圆点代表本次研究所用种群的采样点位，相关信息详见补充表S1。需说明的是，本研究未获取到意大利真螈（*L. italicus*）与兰茨真螈（*L. lantzi*）的相关实验材料。
<br><b>补充图S2</b>
以第一主成分轴（PC1）与第三主成分轴（PC3）可视化的真螈属（Lissotriton）各（亚）种沿躯干区域的形状变化表型轨迹。本次共对9个（亚）种计算了轨迹，其中瑞士真螈（*L. helveticus*）的3个种群单独开展分析。类群按图例所示以颜色区分。连接的圆点代表从T1至T12的平均椎骨形状，以法国马尔西耶拉维尔的瑞士真螈种群为例进行了标注。三维表面模型可直观展示对应的形状变化。
<br><b>补充图S2_3D</b>
展示真螈属9个（亚）种沿躯干区域形状变化表型轨迹的三维图形。请使用谷歌Chrome、火狐浏览器等网页浏览器打开该文件，即可在由第一、第二、第三主成分轴（PC1、PC2和PC3）定义的三维形态空间中可视化轨迹。