Genome-wide transcriptome analysis of pluripotent (2iL) or differentiated (2iL withdrawal) wild-type or Esrrb KO mES cells.

We report the application of genome-wide RNA-sequencing analysis for pluripotent wild-type or Esrrb KO mouse ES cells, maintained in 2iL or differentiated for 24, 48 or 72 hours. We find an anticipated progression of Esrrb KO cells as compared to wild-type cells and a massive downregulation of formative genes in multiple Esrrb KO clones, confirming the role of Esrrb as an inducer of the formative program. Such Esrrb KO cells also show upregulation of committed genes and activation of the Mesendoderm program. mRNA profiles of wild-type and Esrrb KO mES cells cultured in 2iL or after 24, 48 or 72 hours of differentiation.

本研究报道了全基因组RNA测序分析在多能野生型或Esrrb敲除（Esrrb KO）小鼠ES细胞中的应用：这些细胞以2iL培养体系维持未分化状态，或经诱导分化24、48或72小时。相较于野生型细胞，Esrrb敲除细胞呈现出预期的分化进程；且在多个Esrrb敲除细胞克隆中，形成态多能性相关基因出现显著下调，这证实了Esrrb作为形成态多能性程序诱导因子的功能。此类Esrrb敲除细胞同时表现出定向分化基因的上调，以及中内胚层谱系程序的激活。本数据集包含在2iL培养体系中培养的野生型与Esrrb敲除小鼠ES细胞，以及经诱导分化24、48或72小时后的上述细胞的mRNA表达谱。